![](https://parts.igem.org/images/partbypart/icon_rna.png)
RNA
Part:BBa_K4061095:Design
Designed by: Diya Agrawal Group: iGEM21_HKUST (2021-10-15)
mRFP1 antisense characterisation
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1085 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1077
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The antisense mRNA spans 22nt of the protein CDS, the BBa_B0034 RBS and the -10 region of the Constitutive promoter. It also contains an Hfq-binding domain on the 3' end. The promoter region is not necessary for an antisense mRNA to function, however, we included this segment to ensure efficient suppression in case we misjudge the Trascription Start Site.
Source
Biobricks and self-design