RNA

Part:BBa_K4061095:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-15)


mRFP1 antisense characterisation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1085
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1077
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 616
    Illegal AgeI site found at 728
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The antisense mRNA spans 22nt of the protein CDS, the BBa_B0034 RBS and the -10 region of the Constitutive promoter. It also contains an Hfq-binding domain on the 3' end. The promoter region is not necessary for an antisense mRNA to function, however, we included this segment to ensure efficient suppression in case we misjudge the Trascription Start Site.


Source

Biobricks and self-design

References